Cheese.
To make cheese, milk must be curdled until it is reaches a thick, fluid consistency. In the production of hard cheeses, this first step is triggered by the enzyme chymosin. Chymosin is added to milk and breaks down milk proteins causing them to clump together and separate from the watery whey. Traditionally, this enzyme preparation, sometimes known as rennin, was extracted from frozen calf stomachs. The active ingredient is chymosin, an enzyme produced in the stomach of suckling calves needed for breaking down cow’s milk. It is now possible to produce chymosin in genetically modified fungi. These modified microorganisms contain the gene derived from the stomach of calves that is responsible for producing chymosin.

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When grown in a bioreactor, they release chymosin into the culture medium. Afterwards, the enzyme is extracted and purified yielding a product that is 80 to 90 percent pure. Natural rennin contains only 4 to 8 percent active enzyme. Chymosin produced by genetically engineered microorganisms is now used to produce cheese in many different countries. Various milk products may contain additives that are often produced by genetically modified microorganisms. For example beta-carotene coloring (E 160a) used as a yellow dye in butter during the winter - also used in some dairy desserts and yogurt, riboflavin coloring (E 101: Vitamin B2); used in cheeses and cream products, preservatives: Natamycin (E 235), Nisin (E 234), Lysozyme (E 1105); approved for use in cheeses. Dairy desserts, creams, and puddings sometimes contain emulsifiers and thickeners made from GM soybeans or GM maize.


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